The objective of this study was to develop and validate a method for quantitative analysis of rivastigmine hydrogen tartrate (RHT)\nin dual-ligand polymeric nanoparticle formulation matrices, drug release medium, and cellular transport medium. An isocratic\nHPLC analysis method using a reverse phase C18 column and a simple mobile phase without buffer was developed, optimised, and\nfully validated. Analyses were carried out at a flow rate of 1.5 mL/min at 50�°C and monitored at 214 nm. This HPLC method\nexhibited good linearity, accuracy, and selectivity. The recovery (accuracy) of RHTfrom all matrices was greater than 99.2%. The\nRHTpeak detected in the samples of a forced degradation study, drug loading study, release study, and cellular transport study was\npure and free of matrix interference. The limit of detection (LOD) and limit of quantification (LOQ) of the assay were 60 ng/mL\nand 201 ng/mL, respectively. The method was rugged with good intra- and interday precision. This stability indicating HPLC\nmethod was selective, accurate, and precise for analysing RHT loading and its stability in nanoparticle formulation, RHTrelease,\nand cell transport medium.
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